From this the weight-average molecular weight and second virial coefficient can be calculated to provide a measure of protein solubility. The scattering intensity at a range of concentrations is measured to construct a Debye plot. The use of measurements at the stationary layer is one method that is discussed in the next section. There are a number of ways of accounting for this effect.
Static Light Scattering (SLS) is used to determine the molecular weight (> ~1KDa) of proteins and polymers. The Malvern Zetasizer uses electrophoresis, however electroosmosis is an interfering effect that must be considered (Fig.
Malvern zetasizer intensity vs number software#
An electric field is applied to the sample and mixed mode measurement, phase analysis light scattering (M3-PALS) is used to measure electrophoretic mobility and determine a zeta potential distribution. Zetasizer Nano System The Zetasizer Nano system from Malvern Instruments is the first commercial instrument to include the hardware and software for combined dynamic, static, and electrophoretic light scattering measurements, providing the researcher with a wide range of sample properties, including the size, molecular weight, and zeta potential. Non-invasive back-scatter (NIBS) technology is incorporated to give better sensitivity and the ability to measure particles at high concentration.Įlectrophoretic Light scattering (ELS) is used to measure zeta potential. The Zetasizer Nano is a combined light scattering system suitable for characterisation of colloid dispersions, nanoparticles and macromolecules such as polymer and protein solutions (3.8 nm to 100 μm, concentration ~0.1ppm to 40%w/v, pH 1-13, sample dependent).ĭynamic Light Scattering (DLS) is used to measure the diffusion of particles under Brownian motion, and convert this to a size distribution using the Stokes-Einstein relationship. Press the 'Space' or 'Enter' key to toggle the Partner navigation The comprehensive analysis software calculates particle size distributions based on scattering intensity, volume, and number density as well as polydispersity. For typical laboratory samples, this increase can amount to as much as a. The Zetasizer Nano ZS is a static, dynamic, and electrophoretic light scattering instrument, and is the first commercial system to combine the technologies for accurate and absolute size, mass, and zeta. apparent in the width of the intensity distribution.
Press the 'Space' key to toggle the Partner navigation Partner. The size and mass measurements for the Synapse samples used in this study were collected using a Malvern Panalytical Zetasizer Nano ZS system. Research Collaboration and Partnerships.Research funding and grant opportunities.Research centres, groups and facilities.